Epithelial-Mesenchymal Transition in Colorectal Cancer Cell Lines

The human NEC cell line SS-2 was previously established [6 (link)]. The SS-2 cells and other conventional CRC cell lines, HT-29-Luc (JCRB Cell Bank, Osaka, Japan), DLD-1 (Cell Resource Center for Biomedical Research, Tohoku University), Colo-320 (Riken BRC Cell Bank), and Caco-2 (Riken BRC Cell Bank), were grown in RPMI 1640 medium containing 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in a humidified 5% CO₂ atmosphere. For EMT induction, the cells were cultured in growth medium with 10 ng/mL TGF-β1 (Peprotech, Rocky Hill, NJ, USA) for 48 h.

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Sasaki N., Shinji S., Shichi Y., Ishiwata T., Arai T., Yamada T., Takahashi G., Ohta R., Sonoda H., Matsuda A., Iwai T., Takeda K., Yonaga K., Ueda K., Kuriyama S., Miyasaka T, & Yoshida H. (2022). TGF-β1 increases cellular invasion of colorectal neuroendocrine carcinoma cell line through partial epithelial-mesenchymal transition. Biochemistry and Biophysics Reports, 30, 101239.

Publication 2022

HT-29-Luc Colo-320 Caco-2 RPMI 1640 medium fetal bovine serum TGF-β1

Atmosphere Cell lines Cells Fetal bovine serum Growth medium Ht 29 Human Tgf β1

Corresponding Organization : Nippon Medical School

Other organizations : Tokyo Metropolitan Geriatric Hospital

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Variable analysis

independent variables

TGF-β1 (10 ng/mL)

dependent variables

Epithelial-mesenchymal transition (EMT) induction

control variables

Cell culture medium (RPMI 1640 with 10% fetal bovine serum)
Cell lines (SS-2, HT-29-Luc, DLD-1, Colo-320, Caco-2)
Incubation conditions (37 °C, 5% CO2, humidified atmosphere)

controls

No TGF-β1 treatment (negative control)

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