Multiparametric Immune Cell Profiling

1 × 10⁶ cells were Fc blocked (Biolegend101320) and stained for 1 hour, on ice, in the dark, as previously described.^{20 (link)} The following antibodies were used, B220−FITC (BD553087), I Ab PerCP−Cy5.5 (Biolegend116416), Ly6C PE or PECy7 (Biolegend128008, 128071), HLA−DR PECy7 (eBioscience25−9956−41/Biolegend307606), CD11b BV510 (BD562950/Biolegend101263), Ly6G BV605 (BD563005/Biolegend127639), CD11c APC (BD550261), CD3 A700 (eBioscience56−0032−82/Biolegend100216), Live Blue Fluorescent reactive dye (LifeTech L34962), and CD45 BV650 (Biolegend103151).

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Jankowska−Gan E., Agashe V.V., Lema D.A., Zhou Y., Gonzalez Bosc L., Sullivan J.A., Greenspan D.S, & Burlingham W.J. (2020). Donor HLA−DR Drives the Development of De Novo Autoimmunity Following Lung and Heart Transplantation. Transplantation Direct, 6(10), e607.

Publication 2020

Fc blocked B220−FITC HLA−DR PECy7 CD11b BV510 Ly6G BV605 CD11c APC CD3 A700 CD45 BV650

Antibodies Cd11b Cells Cy5 5 Fitc Fluorescent dye Hla dr

Corresponding Organization : University of Wisconsin–Madison

Other organizations : University of New Mexico

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Variable analysis

independent variables

Fc block (Biolegend101320)

dependent variables

B220−FITC (BD553087)
I Ab PerCP−Cy5.5 (Biolegend116416)
Ly6C PE or PECy7 (Biolegend128008, 128071)
HLA−DR PECy7 (eBioscience25−9956−41/Biolegend307606)
CD11b BV510 (BD562950/Biolegend101263)
Ly6G BV605 (BD563005/Biolegend127639)
CD11c APC (BD550261)
CD3 A700 (eBioscience56−0032−82/Biolegend100216)
Live Blue Fluorescent reactive dye (LifeTech L34962)
CD45 BV650 (Biolegend103151)

control variables

Cell number (1 × 10^6 cells)
Staining duration (1 hour)
Staining temperature (on ice)
Staining conditions (in the dark)

positive control

Not explicitly mentioned

negative control

Not explicitly mentioned

Annotations

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