Psoriasis-like Dermatitis Model in Mice

All mice used in this study were purchased from GemPharmatech (Nanjing, Jiangsu, China) including C57BL/6JGpt mice (wild type, WT), B6/JGptGsdme^em8Cd566/Gpt (Gsdme^−/− mice), Krt14^Cre/+-Gsdme^fl/fl (keratinocyte-specific Gsdme cKO mice) and their littermate controls (Krt14 + /+-Gsdme^fl/fl). All mice in experiments were between 6-8 weeks of age and weighted 15-25 g. All mice in our experiment were male mice. All mice were randomly allocated to different groups. All mice were applied with imiquimod cream (Sichuan MingXin Pharmaceutical Co. China) on dorsal skin to establish a psoriasis-like dermatitis model. All mice were sacrificed after IMQ applied for 5 consecutive days, and their tissues were collected. No mice were excluded from the analysis. The severity of skin lesions in mice was evaluated by PASI scores, as detailed in our previous study [9 (link)]. Two investigators conducted a blind assessment of the severity of skin lesions in mice.