Erastin-Induced Tardigrade Tun Formation

To determine the role of VDAC2 on tardigrade tun formation, erastin inhibition was conducted using 3 replicates of 30 tardigrades, each tardigrade in an individual well of a 96-well plate. Tardigrades were incubated in a 100 nM working concentration of erastin (MedChemExpress, Monmouth Junction, NJ) in 0.1% DMSO overnight. After 20 h, tardigrades were observed under a dissecting light microscope and manually counted to determine the number of tuns formed.

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Smythers A.L., Joseph K.M., O’Dell H.M., Clark T.A., Crislip J.R., Flinn B.B., Daughtridge M.H., Stair E.R., Mubarek S.N., Lewis H.C., Salas A.A., Hnilica M.E., Kolling D.R, & Hicks L.M. (2024). Chemobiosis reveals tardigrade tun formation is dependent on reversible cysteine oxidation. PLOS ONE, 19(1), e0295062.

Publication 2024

erastin

Corresponding Organization : Marshall University

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Variable analysis

independent variables

Erastin inhibition

dependent variables

Number of tuns formed

control variables

100 nM working concentration of erastin
0.1% DMSO
Incubation time of 20 hours
30 tardigrades per replicate
Tardigrades in individual wells of a 96-well plate

controls

No positive control mentioned
No negative control mentioned

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