RayBio® Human Biotin Label Based Antibody Arrays - Human L-507 Array, Membrane (AAH-BLM-1A-2, RayBio®, US) was used to analyze the supernatant (conditioned media) of Huh-7 cells (control or macroH2A1 KD), according to manufacturer's instructions. A Human Cytokines antibody array membrane (Abcam, Germany) was used to analyze the supernatant (conditioned media) of HepG2 cells (control or macroH2A1 KD), according to manufacturer's instructions (ab133997, Abcam, US). Detection of IL-6 and IL-8 levels in the culture media of Huh-7 cells was performed using Quantikine® kits (Bio-Techne R&D Systems s.r.o., Prague, Czech Republic), according to manufacturer's instructions.
Nuclei protein fractions from HepG2 and Huh-7 CTL cells were isolated as previously described 23 (link), 24 (link). Primary antibodies were obtained from Active Motif (macroH2A1.1 and macroH2A1.2) and Cell Signaling Technology (H2B).
Nuclei protein fractions from HepG2 and Huh-7 CTL cells were isolated as previously described 23 (link), 24 (link). Primary antibodies were obtained from Active Motif (macroH2A1.1 and macroH2A1.2) and Cell Signaling Technology (H2B).
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