Solid passage rates for extruded flaxseed, Salmate®, and control cows were estimated using chromium oxide (Cr2O3) [31 (link)]. Each cow was administered 14 g of Cr2O3 in a single dose through a ruminal fistula. Fecal samples were collected directly from the rectum after dosing with Cr2O3 dose. Fecal samples were dried in an oven at 55.0°C (72:0 h), milled (1.0 mm), and stored for analysis of Cr2O3 content using spectrophotometry at 400:0 μm and calculated from a standard curve [32 ].
Liquid passage rates for extruded flaxseed, Salmate®, and control cows were estimated using polyethylene glycol. Each cow was administered 100 g of polyethylene glycol through a ruminal fistula (diluted in 200 mL of distilled water). Polyethylene glycol concentrations in the samples were determined in ruminal fluids using gas chromatography.
Liquid passage rates for extruded flaxseed, Salmate®, and control cows were estimated using polyethylene glycol. Each cow was administered 100 g of polyethylene glycol through a ruminal fistula (diluted in 200 mL of distilled water). Polyethylene glycol concentrations in the samples were determined in ruminal fluids using gas chromatography.
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