NPC Immunostaining: Detailed Protocol

NPC immunostaining was performed according to previously published protocols (26 (link), 29 (link)). Primary antibodies used were anti-Nestin (clone 10C2; Merck Millipore, Billerica, MA), anti-TUJ1 (beta III tubulin; clone TUJ1 Covance Inc. San Diego, CA), anti-Vimentin (clone V9; Merck Millipore, Billerica, MA), anti-Oct3/4 (clone H134, Santa Cruz Biotechnology, Inc. Dallas, TX), anti-Nanog (Clone H-155 Santa Cruz Biotechnology Inc. Dallas, TX).

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Chow L., McGrath S., de Arruda Saldanha C., Whalen L.R., Packer R, & Dow S. (2020). Generation of Neural Progenitor Cells From Canine Induced Pluripotent Stem Cells and Preliminary Safety Test in Dogs With Spontaneous Spinal Cord Injuries. Frontiers in Veterinary Science, 7, 575938.

Publication 2020

anti-Vimentin (clone V9; anti-Oct3/4 (clone H134, anti-Nanog (Clone H-155

Antibodies Beta tubulin Clone Nestin Oct3 Vimentin

Corresponding Organization : Colorado State University

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Variable analysis

independent variables

Primary antibodies used were anti-Nestin (clone 10C2; Merck Millipore, Billerica, MA), anti-TUJ1 (beta III tubulin; clone TUJ1 Covance Inc. San Diego, CA), anti-Vimentin (clone V9; Merck Millipore, Billerica, MA), anti-Oct3/4 (clone H134, Santa Cruz Biotechnology, Inc. Dallas, TX), anti-Nanog (Clone H-155 Santa Cruz Biotechnology Inc. Dallas, TX)

dependent variables

NPC immunostaining

control variables

Previously published protocols (26 (link), 29 (link))

Annotations

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