Assessing H. pylori Viability via Live/Dead Staining

To determine the viability of H. pylori strains when assessing the survival at specific time points, the study was expanded to include fluorescence analysis using Live/Dead staining kit (L7012, ThermoFisher, Waltham, MA, USA) [38 (link)]. Slides were viewed under an Olympus BX51 microscope (Olympus Optical, Tokyo, Japan), working with a ×10 lens. Using the ImageJ, the green and red fluorescence of regions of interests (ROIs) (50 ROIs/tested sample) was determined and presented as the mean green/red fluorescence ratio. The fluorescence intensity of SYTO9 (green fluorescent dye) and propidium iodide (red fluorescent dye) was measured at emission levels of 530 nm and 640 nm, respectively.