Locomotor Activity and Anxiety Assay

Locomotor activity and anxiety was assessed using Noldus PhenoTyper cages as previously shown^{58 (link)}. Each cage is outfitted with two sets of cameras; one on the ceiling that faces the platform (35 cm × 35 cm) and another pointed at the side of the cage. Mice were acclimated to the experimentation room in their home cages for at least 1 h. During acclimation, the Noldus EthoVision software was set-up to track movement for 30 total minutes. After acclimation, mice were placed in the center of the open field, opaque Plexiglas was placed on all four sides of the cage to obscure any visual cues, and the trial was started in the EthoVision software. After 30 min, the trial ended and mice were placed back into their home cages. A separate cohort of mice received systemic (I.P.) injections of PF-04531083 (40 mg/kg) or PF-06305591 (2 mg/kg) 1.5 h prior to placement in the novel open field. Noldus EthoVision software was used to determine distance traveled, time spent in center, and frequency of going to center.