Transcriptomic Analysis of Transgenic Tobacco

Total RNAs from the sixth leaves of wild type and EsMYB90 transgenic tobacco at the 7–8 leaf stage were isolated using a Quick RNA isolation kit (Bioteke Corporation, Beijing, China). The RNA library construction and sequencing were performed in the BGI Corporation (Shenzhen, China) using the BGISEQ-500 platform. Three independent biological replicates were carried out for the leaves of wild type and EsMYB90 transgenic tobacco, respectively. The methods of getting the clean reads, gene functional annotation, identification of differential expressed genes, and GO and KEGG pathway functional enrichment analysis has been reported and shown in our previous publication [37 (link)]. The DEGs with log₂Fold Change ≥1 (or ≤−1) and Padj ≤ 0.05 were designated as significantly differentially expressed genes.