Evaluating Cellular Response to Panobinostat

The xCELLigence RTCA SP System (ACEA Biosciences Inc.) was used to assess the real-time analysis of the cellular response of A375, HeLa, HepG2, and Huh7 cell lines (25 (link)–27 (link)) to panobinostat treatment. 50ul of complete media was used to blank the 96X E-Plates, and thereafter, 10⁴ cell/50ul (per well) were seeded into the 96X E-Plates and monitored with impedance-based xCELLigence System until a log growth forming a monolayer is obtained (approximately 18-24 hours). Increasing concentration of panobinostat (25, 50, and 100nM) or DMSO control was then added into each well to make 200ul complete media. The cell index (CI) was measured continuously following ≥70 hours of incubation. CI was normalized at the end of the experiment, and it represents several cellular indicators such as cell-cell attachment, cell number, or cell size, and increased CI indicates cell growth.

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Afolabi L.O., Bi J., Li X., Adeshakin A.O., Adeshakin F.O., Wu H., Yan D., Chen L, & Wan X. (2021). Synergistic Tumor Cytolysis by NK Cells in Combination With a Pan-HDAC Inhibitor, Panobinostat. Frontiers in Immunology, 12, 701671.

Publication 2021

xCELLigence RTCA SP System

Cell Cell lines Cellular attachment Dmso Hela Panobinostat Rtca

Corresponding Organization : University of Chinese Academy of Sciences

Other organizations : Shenzhen University

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Variable analysis

independent variables

Concentration of panobinostat (25, 50, and 100nM)

dependent variables

Cell index (CI), which represents several cellular indicators such as cell-cell attachment, cell number, or cell size

control variables

Complete media used to blank the 96X E-Plates
Cell density (10^4 cells/50ul per well) seeded into the 96X E-Plates
DMSO as a control treatment

controls

DMSO control

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