Transcriptomic Analysis of hCMEC/D3 Cells

hCMEC/D3 cells were grown on 6-well plates coated with calf-skin collagen (Sigma–Aldrich, Gillingham, UK) and collected in TRIzol (Thermo-Fisher Scientific, UK) as described previously [2 (link)]. Total RNA was extracted using a TRIzol Plus RNA purification kit (Thermo-Fisher Scientific, UK) and quantified using a CLARIOstar spectrophotometer equipped with an LVis microplate (BMG Labtech GmbH, Germany).
Hybridization experiments were performed by Macrogen Inc. (Seoul, Republic of Korea) using Illumina HumanHT-12 v4.0 Expression BeadChips (Illumina Inc., San Diego, CA) as described previously [2 (link)].

Free full text: Click here

Hoyles L., Pontifex M.G., Rodriguez-Ramiro I., Anis-Alavi M.A., Jelane K.S., Snelling T., Solito E., Fonseca S., Carvalho A.L., Carding S.R., Müller M., Glen R.C., Vauzour D, & McArthur S. (2021). Regulation of blood–brain barrier integrity by microbiome-associated methylamines and cognition by trimethylamine N-oxide. Microbiome, 9, 235.

Publication 2021

calf-skin collagen TRIzol TRIzol Plus RNA purification kit CLARIOstar spectrophotometer LVis microplate Illumina HumanHT-12 v4.0 Expression BeadChips

Cells Collagen Hybridization Skin Trizol

Corresponding Organization : Nottingham Trent University

Other organizations : University of East Anglia, Queen Mary University of London, Imperial College London, William Harvey Research Institute, Quadram Institute, Norwich Research Park

Top 5 similar protocols

Variable analysis

independent variables

Coating of 6-well plates with calf-skin collagen (Sigma–Aldrich, Gillingham, UK)

dependent variables

Gene expression measured using Illumina HumanHT-12 v4.0 Expression BeadChips (Illumina Inc., San Diego, CA)

control variables

HCMEC/D3 cells were grown on 6-well plates
Total RNA was extracted using a TRIzol Plus RNA purification kit (Thermo-Fisher Scientific, UK)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!