Imaging and Analysis of Fixed Biological Samples

The images of the fixed samples were acquired using inverted SP5 confocal microscope (Leica Microsystems, Manheim, Germany) and Leica 1.3 NA 40X oil (HC PL APO), Leica 1.4 NA 63X oil (HC PL APO) and Leica 1.3 NA 63x glycerol (HCX PL APO) objectives. The images were processed using Fiji (http://fiji.sc), Chimera (https://www.cgl.ucsf.edu/chimera/) and MovIT visualization software (BioEmergences, http://bioemergences.iscpif.fr/)33 (link).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Shahbazi M.N., Jedrusik A., Vuoristo S., Recher G., Hupalowska A., Bolton V., Fogarty N.N., Campbell A., Devito L., Ilic D., Khalaf Y., Niakan K.K., Fishel S, & Zernicka-Goetz M. (2016). Self-organisation of the human embryo in the absence of maternal tissues. Nature cell biology, 18(6), 700-708.

Publication 2016

inverted SP5 confocal microscope Leica 1.3 NA 40X oil Leica 1.4 NA 63X oil Leica 1.3 NA 63x glycerol

Chimera Confocal microscope Glycerol

Corresponding Organization :

Other organizations : Karolinska Institutet, University of Helsinki, University of Cambridge, Laboratoire Photonique, Numérique et Nanosciences, Guy's Hospital, The Francis Crick Institute

Top 5 similar protocols

Variable analysis

independent variables

Microscope type (inverted SP5 confocal microscope)

dependent variables

Images of the fixed samples

control variables

Leica 1.3 NA 40X oil (HC PL APO) objective
Leica 1.4 NA 63X oil (HC PL APO) objective
Leica 1.3 NA 63x glycerol (HCX PL APO) objective

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!