The c-Maf and Otub1 plasmids were subcloned into a pcDNA3.1 vector carrying an HA, or Myc tag. The luciferase reporter driven by c-Maf recognition element (MARE) (5′-TGCGAGTGAGGCA-3′) (pGL4-MARE.Luci) was synthesized by GeneWiz, Inc. (Suzhou, Jiangsu, China) [7 (link)]. The Otub1-siRNA and control-siRNA were purchased from Ribobio Inc. (Guangzhou, China) [11 ].
The antibodies used for Western blot were as follows: anti-Otub1 and anti-Ub were from Santa Cruz Biotechnology Co. Ltd (Santa Cruz, CA); anti-glyceraldehyde-3-phosphatedehydrogenase (GAPDH), anti-integrin beta 7 (ITGB7) and anti-c-Maf were from Proteintech Group, Inc. (Wuhan, China); anti-HA and anti-Myc were obtained from MBL Biotech Co., (Beijing, China); anti-poly(ADP-ribose) polymerase (PARP), anti-Caspase 3 and anti-cyclin D2 (CCND2) were purchased from Cell Signaling Technology (Danvers, MA). Antibodies of Otud4, Otud5, and Otud7b were provided by Beyotime Institute of Biotechnology (Haimen, China).
The antibodies used for Western blot were as follows: anti-Otub1 and anti-Ub were from Santa Cruz Biotechnology Co. Ltd (Santa Cruz, CA); anti-glyceraldehyde-3-phosphatedehydrogenase (GAPDH), anti-integrin beta 7 (ITGB7) and anti-c-Maf were from Proteintech Group, Inc. (Wuhan, China); anti-HA and anti-Myc were obtained from MBL Biotech Co., (Beijing, China); anti-poly(ADP-ribose) polymerase (PARP), anti-Caspase 3 and anti-cyclin D2 (CCND2) were purchased from Cell Signaling Technology (Danvers, MA). Antibodies of Otud4, Otud5, and Otud7b were provided by Beyotime Institute of Biotechnology (Haimen, China).
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