At baseline and after six weeks, we assessed the renal function through the evaluation of creatinine and estimated glomerular filtration rate (e-GFR). At the same time-points we evaluated the inflammatory status with C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR). All patients underwent urinalysis to check UTIs signs. Furthermore, Free Oxygen Radical Test (FORT) and Free Oxygen Radical Defense (FORD) test were performed by CR4000, on capillary blood samples, to evaluate the oxidative stress [46 (link)] and the total antioxidant defense capacity [47 (link)], respectively.
A Dimension Vista 1500 (Siemens Healthcare Diagnostics, Milano, Italy) instrument was used to monitor all parameters. Standard enzymatic colorimetric techniques (Roche Modular P800, Roche Diagnostics, Indianapolis, IN, USA) were used to assess the lipid profile.
All other parameters were analyzed according to standard procedures of Clinical Biochemical Laboratories of University Hospital PTV of Rome.
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