Metagenomic DNA Extraction and Quantification

Metagenomic DNA extraction was then performed on the frozen pellets via chemical and mechanical lysis using a Pathogen Lysis Tube S and QIAamp UCP Pathogen Mini Kit (Qiagen, Hilden, Germany) [22 (link),23 ,69 ]. Subsequently, quantification of the purified genomic DNA was performed using a Quantus fluorometer (Promega, Madison, WI, USA) and Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). The quantified DNA was frozen at −80 °C until use.

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Wang D., Nambu T., Tanimoto H., Iwata N., Yoshikawa K., Okinaga T, & Yamamoto K. (2021). Interdental Plaque Microbial Community Changes under In Vitro Violet LED Irradiation. Antibiotics, 10(11), 1348.

Publication 2021

Pathogen Lysis Tube S QIAamp UCP Pathogen Mini Kit Quantus fluorometer Qubit dsDNA BR Assay Kit

Assay Dsdna Frozen Genomic Metagenomic Pathogen Pellets Promega

Corresponding Organization : Osaka Dental University

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Variable analysis

independent variables

Chemical and mechanical lysis using a Pathogen Lysis Tube S and QIAamp UCP Pathogen Mini Kit (Qiagen, Hilden, Germany)

dependent variables

Quantification of the purified genomic DNA using a Quantus fluorometer (Promega, Madison, WI, USA) and Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA)

control variables

Frozen pellets

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