Cultivation and Antibiotic Resistance of S. aureus

The bacterial strains and plasmids used in this study are listed in Table 1. The primers used are listed in Table 2. When necessary, the corresponding antibiotics (100 μg/ml ampicillin and 50 μg/ml kanamycin) were added to the medium. S. aureus was usually cultured in Tryptone Soy Broth medium at 37°C and 220 rpm, to which 15 μg/ml chloramphenicol and 10 μg/ml erythromycin were added when necessary.

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Fu T., Fan Z., Li Y., Li Z., Du B., Liu S., Cui X., Zhang R., Zhao H., Feng Y., Xue G., Cui J., Yan C., Gan L., Feng J., Xu Z., Yu Z., Tian Z., Ding Z., Chen J., Chen Y, & Yuan J. (2023). ArcR contributes to tolerance to fluoroquinolone antibiotics by regulating katA in Staphylococcus aureus. Frontiers in Microbiology, 14, 1106340.

Publication 2023

Ampicillin Antibiotics Bacterial Chloramphenicol Cultured medium Erythromycin Kanamycin Plasmids Primers S aureus Strains

Corresponding Organization : Capital Institute of Pediatrics

Other organizations : University of Science and Technology of China

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Variable analysis

independent variables

Bacterial strains
Plasmids

dependent variables

Growth of S. aureus

control variables

Antibiotics (100 μg/ml ampicillin and 50 μg/ml kanamycin)
Culture conditions (37°C, 220 rpm)
Chloramphenicol (15 μg/ml)
Erythromycin (10 μg/ml)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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