Monitoring Nitric Oxide-Induced Bacterial Switch

SL. pNASR were treated with 100 μM DETA/NO and further cultured at 37 °C. Aliquots were collected after 0–8 h in culture, with the resulting bacterial pellets resuspended in PBS. Rluc8 activity was measured by determining bioluminescence intensities in the presence of coelenterazine, as described above. The switch events were also analyzed by PCR using fimS and rluc8 primers.

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Qin Y., You S.H., Zhang Y., Venu A., Hong Y, & Min J.J. (2023). Genetic Programming by Nitric Oxide-Sensing Gene Switch System in Tumor-Targeting Bacteria. Biosensors, 13(2), 266.

Publication 2023

Bacterial Coelenterazine Deta Pellets Primers

Corresponding Organization : Chonnam National University

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Variable analysis

independent variables

Treatment with 100 μM DETA/NO

dependent variables

Rluc8 activity measured by bioluminescence intensities
Analysis of switch events by PCR using fimS and rluc8 primers

control variables

Culture at 37 °C
Resuspension of bacterial pellets in PBS

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