Antibody Response to DTPa-6 Vaccine

To determine antibody responses elicited by DTPa-6 (Infanrix Hexa; GlaxoSmithKline, Brentford, UK), we performed enzyme-linked immunosorbent assays, as previously described [40 (link)]. In brief, tetanus toxoid (TT), Bordetella pertussis toxoid (PT), Bordetella pertussis pertactic (PRN), and filamentous hemagglutinin (FHA) were coated to plates and overlaid with serially diluted mouse serum, and bound antibodies were detected using a mouse IgG-specific detection antibody, the binding of which was quantified by means of an electrochemiluminescent label.

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Belnoue E., Vogelzang A., Nieuwenhuizen N.E., Krzyzaniak M.A., Darbre S., Kreutzfeldt M., Wagner I., Merkler D., Lambert P.H., Kaufmann S.H., Siegrist C.A, & Pinschewer D.D. (2022). Replication-Deficient Lymphocytic Choriomeningitis Virus-Vectored Vaccine Candidate for the Induction of T Cell Immunity against Mycobacterium tuberculosis. International Journal of Molecular Sciences, 23(5), 2700.

Publication 2022

Infanrix Hexa

Antibodies Antibody responses Bordetella Bordetella pertussis Dtpa Enzyme linked immunosorbent assays Filamentous Hemagglutinin Igg antibody Infanrix hexa Mouse Pertussis toxoid Serum Tetanus toxoid Toxoid

Corresponding Organization : University of Basel

Other organizations : Max Planck Institute for Infection Biology, University Hospital of Geneva

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Variable analysis

independent variables

Tetanus toxoid (TT)
Bordetella pertussis toxoid (PT)
Bordetella pertussis pertactic (PRN)
Filamentous hemagglutinin (FHA)

dependent variables

Antibody responses elicited by DTPa-6 (Infanrix Hexa)

control variables

Serially diluted mouse serum
Mouse IgG-specific detection antibody
Electrochemiluminescent label

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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