Immunohistochemical Characterization of Axon Initial Segment

All chemical and reagents were purchased from Sigma-Aldrich, unless otherwise specified. Antisera used, which were previously described, include rabbit anti-AnkG, anti–βIV Spectrin, and anti–pan Na_V Channels (Taylor et al., 2017 (link)), guinea pig and rabbit anti-Caspr (Bhat et al., 2001 (link)), guinea pig anti-NF186 (Thaxton et al., 2011 (link)), and rat anti-NFCT (Pillai et al., 2009 (link)). Primary antibodies include rabbit anti-Gapdh (RRID:AB_796208, #G9545); mouse anti–β-actin (RRID:AB_476744, #A-5441); mouse anti-Caspr (RRID:AB_2083496, #75-001, NeuroMab); anti-AnkR (RRID:AB_2491109, #75-380, NeuroMab); and anti-Kv1.2 (RRID:AB_2296313, #75-008, NeuroMab). Fluorescent secondary antibodies (Alexa Fluor; Life Technologies) and infrared (IR)-conjugated secondary antibodies (LI-COR) were also purchased. Reagents used to perform electron microscopy were from Electron Microscopy Sciences.

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Taylor A.M., Shi Q, & Bhat M.A. (2018). Simultaneous Ablation of Neuronal Neurofascin and Ankyrin G in Young and Adult Mice Reveals Age-Dependent Increase in Nodal Stability in Myelinated Axons and Differential Effects on the Lifespan. eNeuro, 5(3), ENEURO.0138-18.2018.

Publication 2018

IR)-conjugated secondary antibodies

Actin Antibodies Antisera Caspr Electron microscopy Fluorescent antibodies Gapdh Guinea pig Mouse Rabbit Spectrin

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