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Extracellular Vesicle Purification Protocol

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Hoshino A., Kim H.S., Bojmar L., Gyan K.E., Cioffi M., Hernandez J., Zambirinis C.P., Rodrigues G., Molina H., Heissel S., Mark M.T., Steiner L., Benito-Martin A., Lucotti S., Giannatale A.D., Offer K., Nakajima M., Williams C., Nogués L., Vatter F.A., Hashimoto A., Davies A.E., Freitas D., Kenific C.M., Ararso Y., Buehring W., Lauritzen P., Ogitani Y., Sugiura K., Takahashi N., Alečković M., Bailey K.A., Jolissaint J.S., Wang H., Harris A., Schaeffer L.M., García-Santos G., Posner Z., Balachandran V.P., Khakoo Y., Raju G.P., Scherz A., Sagi I., Scherz-Shouval R., Yarden Y., Oren M., Malladi M., Petriccione M., De Braganca K.C., Donzelli M., Fischer C., Vitolano S., Wright G.P., Ganshaw L., Marrano M., Ahmed A., DeStefano J., Danzer E., Roehrl M.H., Lacayo N.J., Vincent C.T., Weiser M.R., Brady M.S., Meyers P.A., Wexler L.H., Ambati S.R., Chou A.J., Slotkin E.K., Modak S., Roberts S.S., Basu E.M., Diolaiti D., Krantz B.A., Cardoso F., Simpson A.L., Berger M., Rudin C.M., Simeone D.M., Jain M., Ghajar C.M., Batra S.K., Stanger B.Z., Bui J., Brown K.A., Rajasekhar V.K., Healey J.H., de Sousa M., Kramer K., Sheth S., Baisch J., Pascual V., Heaton T.E., Quaglia M.P., Pisapia D.J., Schwartz R., Zhang H., Liu Y., Shukla A., Blavier L., DeClerck Y.A., LaBarge M., Bissell M.J., Caffrey T.C., Grandgenett P.M., Hollingsworth M.A., Bromberg J., Costa-Silva B., Peinado H., Kang Y., Garcia B.A., O’Reilly E.M., Kelsen D., Trippett T.M., Jones D.R., Matei I.R., Jarnagin W.R, & Lyden D. (2020). Extracellular Vesicle and Particle Biomarkers Define Multiple Human Cancers. Cell, 182(4), 1044-1061.e18.

Publication 2020

Adipose Apoptotic bodies Cell Cell culture Cell lines Centrifugation Conditioned media Deuterium oxide Isolation Lm10 Mass spectrometry Microvesicles Pellets Protein Sucrose Tissue Tris Ultracentrifugation Violet

Corresponding Organization : Memorial Sloan Kettering Cancer Center

Other organizations : Yonsei University, Linköping University, Uppsala University, Tri-Institutional PhD Program in Chemical Biology, National Institutes of Health, National Cancer Institute, Universidade do Porto, Rockefeller University, École Polytechnique Fédérale de Lausanne, Bambino Gesù Children's Hospital, Istituti di Ricovero e Cura a Carattere Scientifico, Spanish National Cancer Research Centre, Centro de Investigación del Cáncer, The University of Tokyo, The Ohio State University, i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Princeton University, Central University Hospital of Asturias, Icahn School of Medicine at Mount Sinai, Weizmann Institute of Science, Lucile Packard Children's Hospital, New York University, NYU Langone Health, Champalimaud Foundation, University of Nebraska Medical Center, Fred Hutch Cancer Center, University of Pennsylvania, University of California, San Diego, University of Vermont, University of Southern California, City of Hope, Lawrence Berkeley National Laboratory

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