Western blots were performed on SDS protein lysates quantified by picogreen assay, as described10 (link). Lysates that contained 10,000 cells were resolved on SDS polyacrylamide gels, before transferring onto PVDF membranes and probing with primary antibodies overnight at 4 °C. Antibodies used: VCAM1 (AF643, R&D), THMB (Ab109189, Abcam), HMOX1 (Ab52947, Abcam), NFκB (#8242, Cell Signalling), NFκB pSer536 (#3033, Cell Signalling).
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