Cells were irradiated with various doses of 6 MV X-rays from a conventional linear accelerator (Versa HDTM, Elekta AB, Stockholm, Sweden). Irradiation was performed in T25 flasks (colony formation assay) with a field setup published previously [54 (link)]. In combined modality treatments (together with Vpr or TMZ) or control treatments (DMSO), irradiation was performed 6 hours after the addition of the corresponding substance.
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