Two-way analysis of variance (ANOVA) with repeated measures followed by Bonferroni
post-hoc test was used to analyze the time course of mechanical hyperalgesia, according to
condition (i.e., sham/CCI-ION) and time as independent factors. Two-way ANOVA followed by
Bonferroni post-hoc test was performed to analyze the USV emission after d,l-amphetamine
treatment. Analyzes of western blot and immunofluorescence intensity were performed using
the unpaired two-tailed Student’s t-test. Sample size was determined based on the GPower
3.1 software31 (link)
defining a large standardized effect size of F = 0.5; power of 0.8; and α = 0.05,
and estimated six to eight rats per group in the different experimental designs. For c-Fos
detection, the sample size was based on previous studies32 (link),33 (link) to recruit the smallest number of rats.
All data were expressed as mean ± SEM, and the results were considered statistically
significant if p < 0.05. GraphPad Prism version 6 for Windows was used
for statistical analysis of data (GraphPad Software, San Diego, CA).
post-hoc test was used to analyze the time course of mechanical hyperalgesia, according to
condition (i.e., sham/CCI-ION) and time as independent factors. Two-way ANOVA followed by
Bonferroni post-hoc test was performed to analyze the USV emission after d,l-amphetamine
treatment. Analyzes of western blot and immunofluorescence intensity were performed using
the unpaired two-tailed Student’s t-test. Sample size was determined based on the GPower
3.1 software31 (link)
defining a large standardized effect size of F = 0.5; power of 0.8; and α = 0.05,
and estimated six to eight rats per group in the different experimental designs. For c-Fos
detection, the sample size was based on previous studies32 (link),33 (link) to recruit the smallest number of rats.
All data were expressed as mean ± SEM, and the results were considered statistically
significant if p < 0.05. GraphPad Prism version 6 for Windows was used
for statistical analysis of data (GraphPad Software, San Diego, CA).
