For sequencing of the mitochondrial genomes reported here, mtDNAs were isolated via CsCl-bisbenzimide equilibrium gradient centrifugation, sequenced by the Sanger technology, assembled with Phred/Phrap (28 (link),29 (link)) and annotated with the MFannot tool (http://megasun.bch.umontreal.ca/cgi-bin/mfannot/mfannotInterface.pl), essentially as described earlier (27 (link),30 (link)–31 (link)). Complete mtDNA sequences have been deposited in GenBank under the accession numbers KP165385-KP165391. For transcriptome sequencing, total RNA including small RNAs was extracted from cells using the RNeasy Plus Universal Kit (Qiagen). RNA-Seq libraries were constructed using the TruSeq Small RNA Sample Prep kit (Illumina) following the supplier's instructions, except that total RNA was not size-fractionated. Both the library preparation and the paired-end Illumina sequencing were outsourced to the technology platform of the Genome Quebec Innovation Center in Montreal, generating 18–39 million Illumina HiSeq2500 (up to 150 nt read length for A. godoyi, M. jakobiformis and M. californiana) and 6.8 million Illumina MiSeq paired-end reads (up to 250 nt read length for J. bahamiensis). The applied procedure yields RNA-Seq data that allow accurate mapping of 5S rRNA termini and precise determination of RNA steady-state levels.