Pancreatic Cancer Cell Line Culturing

The MIT Animal Care and Use Committees reviewed and approved all animal studies and procedures. NOD/SCID/IL2Rγ-null mice (Jackson Laboratory) were used throughout the study. Mice were housed on a 12-h light/12-h dark cycle at ~21 °C and 40–60% humidity. The human pancreatic adenocarcinoma (PDAC) cell lines AsPC1, BxPC3, PANC1, and MIAPaCa2 and HEK 293FT cells were purchased from American Type Cell Culture (ATCC), where they were tested and authenticated; PSN1 and CFPAC1 were gifts from the Koch Institute cell line repository, and they were originally tested and authenticated from ATCC. The human CAF cell line hT1 and hM1 were published previously^{59 (link)}. All cells were cultured in media supplemented with 10% fetal bovine serum (FBS, Invitrogen) at 37 °C in a 5% CO2 incubator. AsPC1 was cultured in RPMI medium 1640 (ThermoFisher); 293FT, BxPC3, PANC1, MIAPaCa2 and PSN1 were cultured in Dulbecco’s modified Eagle’s medium (DMEM, ThermoFisher); CFPAC1 was cultured in Iscove’s modified Dulbecco’s Medium (ThermoFisher).