Protein Expression Analysis by Western Blot

Western blot analysis of proteins was conducted as previously described 43 (link), 52 (link). The primary antibodies used were CD206 (1:1000; R&D system), Arginase-1 (Arg-1, 1:500; Santa Cruz), CD63 (1:800; Santa Cruz), TSG101 (1:800; Abcam), Calnexin (1:800; Servicebio), PDGFR-α (1:1000; Santa Cruz), MBP (1:1000; Abcam), β-actin (1:1000; Proteintech, CA), GAPDH (1:1000; Santa Cruz). The semi-quantification of the chemiluminescence signal was calculated using Image J software and all gene band gray value was standardized to the expression of β-actin or GAPDH.

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Li Y., Liu Z., Song Y., Pan J.J., Jiang Y., Shi X., Liu C., Ma Y., Luo L., Mamtilahun M., Shi Z., Khan H., Xie Q., Wang Y., Tang Y., Zhang Z, & Yang G.Y. (2022). M2 microglia-derived extracellular vesicles promote white matter repair and functional recovery via miR-23a-5p after cerebral ischemia in mice. Theranostics, 12(7), 3553-3573.

Publication 2022

CD206 Arginase-1 (Arg-1, TSG101 PDGFR-α β-actin GAPDH

Actin Antibodies Arginase 1 Calnexin Chemiluminescence Gapdh Gene Proteins Tsg101 Western blot analysis

Corresponding Organization :

Other organizations : Ruijin Hospital, Shanghai Jiao Tong University, Renji Hospital, Shanghai Center for Brain Science and Brain-Inspired Technology, Huashan Hospital, Fudan University

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Variable analysis

independent variables

Primary antibodies used: CD206 (1:1000; R&D system), Arginase-1 (Arg-1, 1:500; Santa Cruz), CD63 (1:800; Santa Cruz), TSG101 (1:800; Abcam), Calnexin (1:800; Servicebio), PDGFR-α (1:1000; Santa Cruz), MBP (1:1000; Abcam), β-actin (1:1000; Proteintech, CA), GAPDH (1:1000; Santa Cruz)

dependent variables

Chemiluminescence signal semi-quantification calculated using Image J software

control variables

All gene band gray value was standardized to the expression of β-actin or GAPDH

controls

Positive controls: Not specified
Negative controls: Not specified

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