Automated Antimicrobial Resistance Detection

All samples were examined for AMR patterns through a minimum inhibitory concentration (MIC) - based automated system, the VITEK^® 2 AST-GN80 test kit cards, and the VITEK^® 2 COMPACT machine (bioMérieux) [20 (link), 21 (link)]. The MIC results were interpreted using an advanced Expert System™ based on the global Clinical and Laboratory Standards Institute and natural resistance guidelines, as in a previous study [7 (link)]. The following antimicrobial drugs were included in the VITEK^® 2 AST-GN80 test: Benzylpenicillin, oxacillin, cefalotin (CEF), cefovecin (CEC), ceftiofur (CET), kanamycin (KAN), enrofloxacin (ENR), marbofloxacin (MAR), pradofloxacin, erythromycin, clindamycin (CLI), doxycycline, tetracycline (TCN), nitrofurantoin (NFT), chloramphenicol, and sulfamethoxazole/trimethoprim (SXT). Three AMR genes were re-examined, namely mecA, mecC, and blaZ [21 (link)–23 (link)]. Polymerase chain reaction (PCR) was performed with the primers used in a previous study [7 (link)]. Staphylococcal chromosomal cassette mec gene detection was performed through multiplex PCR [24 (link)].

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Boonchuay K., Sontigun N., Wongtawan T, & Fungwithaya P. (2023). Association of multilocus sequencing types and antimicrobial resistance profiles of methicillin-resistant Mammaliicoccus sciuri in animals in Southern Thailand. Veterinary World, 16(2), 291-295.

Publication 2023

VITEK® 2 AST-GN80 test kit cards VITEK® 2 COMPACT machine

Antimicrobial drugs Benzylpenicillin Cefalotin Cefovecin Ceftiofur Chloramphenicol Chromosomal Clindamycin Clinical laboratory Doxycycline Enrofloxacin Erythromycin Gene Kanamycin Marbofloxacin Meca Mecc Minimum inhibitory concentration Multiplex polymerase chain reaction Natural resistance Nitrofurantoin Oxacillin Polymerase chain reaction Pradofloxacin Primers Staphylococcal Sulfamethoxazole trimethoprim Tetracycline

Corresponding Organization :

Other organizations : Walailak University

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Variable analysis

independent variables

Antimicrobial drugs tested: Benzylpenicillin, oxacillin, cefalotin (CEF), cefovecin (CEC), ceftiofur (CET), kanamycin (KAN), enrofloxacin (ENR), marbofloxacin (MAR), pradofloxacin, erythromycin, clindamycin (CLI), doxycycline, tetracycline (TCN), nitrofurantoin (NFT), chloramphenicol, and sulfamethoxazole/trimethoprim (SXT)

dependent variables

Minimum inhibitory concentration (MIC) values for the antimicrobial drugs
Presence or absence of AMR genes: mecA, mecC, and blaZ

control variables

Global Clinical and Laboratory Standards Institute and natural resistance guidelines used to interpret MIC results
Polymerase chain reaction (PCR) with primers used in a previous study [7] to detect AMR genes
Multiplex PCR to detect Staphylococcal chromosomal cassette mec gene

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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