Cilostazol Modulation of Zebrafish Development

Stock solutions of 20 mg/mL cilostazol (Otsuka Pharmaceutical) were prepared in dimethyl sulfoxide (DMSO, Sigma). Working solutions were freshly prepared by diluting the stock solutions with aerated egg water. The vehicle control contained DMSO at 0.18%. Embryos were placed into these working solutions and cultured at a density of about 30 individuals per 10 ml in a glass beaker. For heart beat measurements, cortisol enzyme-linked immunosorbent assay (ELISA) and whole-mount 3-β-Hydroxysteroid dehydrogenase /Δ5–4 isomerase (3β-Hsd) enzymatic activity assays, the cilostazol treatments commenced immediately after the completion of gastrulation (10 hpf). For the tail fin amputation experiments, the cilostazol treatments were started at 1 day post-fertilization (dpf).

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Chang W.C., Chen M.J., Hsiao C.D., Hu R.Z., Huang Y.S., Chen Y.F., Yang T.H., Tsai G.Y., Chou C.W., Chen R.S., Chuang Y.J, & Liu Y.W. (2023). The anti-platelet drug cilostazol enhances heart rate and interrenal steroidogenesis and exerts a scant effect on innate immune responses in zebrafish. PLOS ONE, 18(10), e0292858.

Publication 2023

DMSO

Amputation Cilostazol Cortisol Dmso Embryos Enzymatic assays Enzyme linked immunosorbent assay Fertilization Gastrulation Heart beat Hydroxysteroid dehydrogenase Isomerase Pharmaceutical Tail

Corresponding Organization :

Other organizations : Tunghai University, Taichung Hospital, Ministry of Health and Welfare, Chung Yuan Christian University, National Tsing Hua University

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Variable analysis

independent variables

Cilostazol concentration

dependent variables

Heart beat measurements
Cortisol levels (measured by ELISA)
3β-Hsd enzymatic activity (whole-mount assay)
Tail fin regeneration

control variables

Embryo density (about 30 individuals per 10 ml)
Vehicle control (DMSO at 0.18%)

controls

Positive control: Not specified
Negative control: Vehicle control (DMSO at 0.18%)

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