Quantitative PCA Extraction and HPLC Detection

PCA must be extracted from the fermentation broth and then detected and quantified by high-performance liquid chromatography (HPLC). We first extracted PCA from the fermentation broth using ethyl acetate and subjected it to reversed-phase C18 column (Agilent Technologies, 5 μm, 4.6*250 mm). Both 2-OH-PHZ and PCN are phenazine derivatives and exhibit similar material properties; the specific extraction and detection steps of these samples are described in our previous studies (Liu et al., 2016 (link); Li et al., 2020 (link)).

Free full text: Click here

Liu K., Li Z., Liang X., Xu Y., Cao Y., Wang R., Li P, & Li L. (2023). Biosynthesis and genetic engineering of phenazine-1-carboxylic acid in Pseudomonas chlororaphis Lzh-T5. Frontiers in Microbiology, 14, 1186052.

Publication 2023

reversed-phase C18 column

Derivatives Ethyl acetate Fermentation High performance liquid chromatography Phenazine

Corresponding Organization : Shandong Academy of Sciences

Other organizations : Dezhou University, Institute of Biophysics

Top 5 similar protocols

Variable analysis

independent variables

Extraction of PCA from the fermentation broth using ethyl acetate
Subjected the extracted PCA to reversed-phase C18 column (Agilent Technologies, 5 μm, 4.6*250 mm)

dependent variables

Detection and quantification of PCA by high-performance liquid chromatography (HPLC)

control variables

2-OH-PHZ and PCN, which are phenazine derivatives and exhibit similar material properties, as described in the previous studies (Liu et al., 2016; Li et al., 2020)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!