Protocol detail

Immunostaining of Stress Granules

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Immunostaining was performed using specific antibodies (Supplementary Table S4) as previously described^{50 (link)}. To detect SGs, cells were treated with arsenite (100 μM; Merck) for 30 min before immunostaining. Cells were counterstained with Hoechst33342, washed five times with PBS, mounted on slides, and imaged by confocal microscopy (Carl Zeiss, Oberkochen, Germany and Olympus, Tokyo, Japan).

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Yoshino S., Matsui Y., Fukui Y., Seki M., Yamaguchi K., Kanamori A., Saitoh Y., Shimamura T., Suzuki Y., Furukawa Y., Kaneko S., Seiki M., Murakami Y., Inoue J.I, & Sakamoto T. (2020). EXOSC9 depletion attenuates P-body formation, stress resistance, and tumorigenicity of cancer cells. Scientific Reports, 10, 9275.

Publication 2020

arsenite confocal microscopy

Antibodies Arsenite Cells Confocal microscopy Hoechst33342

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Variable analysis

independent variables

Arsenite treatment (100 μM; Merck) for 30 min

dependent variables

Formation of Stress Granules (SGs)

control variables

Hoechst33342 counterstaining
Immunostaining with specific antibodies (Supplementary Table S4)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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