Western Blot Analysis of STAT3 and HNF4A

Western blots analyses were performed as described previously [25 (link)] using OF-treated Clone 9 cells, while immunoreactive proteins were detected by an enhanced chemiluminescence kit (Bio-Rad). The specific antibodies against STAT3 (signal transducer and activator of transcription 3), phosphorylated STAT3 (pSTAT3), HNF4A (hepatocyte nuclear factor 4, alpha), and GAPDH (glyceraldehyde 3-phosphate dehydrogenase) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

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Cheng C.C., Yang W.Y., Hsiao M.C., Lin K.H., Lee H.W, & Yuh C.H. (2020). Transcriptomically Revealed Oligo-Fucoidan Enhances the Immune System and Protects Hepatocytes via the ASGPR/STAT3/HNF4A Axis. Biomolecules, 10(6), 898.

Publication 2020

enhanced chemiluminescence kit phosphorylated STAT3 (pSTAT3 HNF4A GAPDH

Antibodies Chemiluminescence Clone cells Gapdh Glyceraldehyde 3 phosphate dehydrogenase Hepatocyte nuclear factor 4 Proteins Stat3 Western blots analyses

Corresponding Organization : Kaohsiung Medical University

Other organizations : Chang Gung University, Linkou Chang Gung Memorial Hospital

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Variable analysis

independent variables

OF-treated Clone 9 cells

dependent variables

STAT3 (signal transducer and activator of transcription 3)
Phosphorylated STAT3 (pSTAT3)
HNF4A (hepatocyte nuclear factor 4, alpha)
GAPDH (glyceraldehyde 3-phosphate dehydrogenase)

control variables

Western blots analyses were performed as described previously [25 (link)]
Immunoreactive proteins were detected by an enhanced chemiluminescence kit (Bio-Rad)
The specific antibodies against STAT3, pSTAT3, HNF4A, and GAPDH were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA)

controls

No positive or negative controls were explicitly mentioned.

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