A mixture of 7-acetyl-intermedine/lycopsamine was from PlantaAnalytica (Danbury, US), retrorsine from Cfm O.Tropitzsch (Marktredwitz, Germany), and echimidine, intermedine, intermedine N-oxide, and senecionine were from PhytoLab (Vestenbergsgreuth, Germany). Echivulgarine was isolated in-house [38 (link)] and echimidine N-oxide and retrorsine N-oxide were N-oxidized in-house, starting from the free bases according to [39 (link)], except that dichloromethane was used instead of trichloromethane.
D3-atropine was from CDN isotopes (Quebec, Canada), methanol was from Honeywell (LC-MS grade, Grogg AG, Stettlen, Switzerland). Sulfuric acid, formic acid, and aqueous ammonia solution 25%, all of analytical grade, were from Merck (Grogg AG, Stettlen, Switzerland). Ultrapure water was used (Elga purelab ultra, Labtec, Villmergen, Switzerland).
D3-atropine was from CDN isotopes (Quebec, Canada), methanol was from Honeywell (LC-MS grade, Grogg AG, Stettlen, Switzerland). Sulfuric acid, formic acid, and aqueous ammonia solution 25%, all of analytical grade, were from Merck (Grogg AG, Stettlen, Switzerland). Ultrapure water was used (Elga purelab ultra, Labtec, Villmergen, Switzerland).
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