Lung Metastasis Suppression by Anti-S100A8/A9

Example 6

Through use of a lung metastasis model of mouse melanoma B16-BL6 cells, the lung metastasis-suppressing effects of anti-S100A8/A9 monoclonal antibodies were investigated. For melanoma, the presence or absence of metastasis can be easily judged by its black color.

In accordance with a protocol illustrated in FIG. 13, 1×10⁵ mouse melanoma B16-BL6 cells and 50 μg of each anti-S100A8/A9 monoclonal antibodies (Clone Nos.: 45, 85, 235, 258, and 260) were simultaneously injected into the tail vein of five Balb/c nu/nu mice per group, and 1 month later, CT scans were performed. FIG. 14 shows the results for comparing typical mouse lung and CT images and areas calculated from the CT images to those of a negative control group. As a result, it was recognized that Clone Nos. 45, 85, 235, and 258 showed significant lung metastasis-suppressing effects. In particular, Clone No. 45 was found to have a strong metastasis-suppressing effect.