CD40xCEA Bispecific Antibody Targeting of Colorectal Tumors

Example 17

Materials and Methods

Human CD40 transgenic mice were inoculated with MC38-hCEA tumor cells (MC-38-CEA-2, Kerafast) s.c. and were administered with 100 μg anti-CD40 antibody or a molar equivalent dose (167 μg) CD40×CEA bsAb (ffAC_05337) or Isotype bsAb i.p. on days 10 and 13. On day 14, tumors were dissected. Frozen tumor sections were stained for human IgG to assess accumulation of administered antibodies, and for CEA to assess CEA expression pattern in the tumors.

Results

FIG. 27 shows accumulation of the CD40×CEA bsAb (ffAC_05337), but not corresponding CD40 mAb, in CEA-expressing tumors