Quantifying Mitochondrial DNA and Citrate Synthase

Mitochondrial DNA (mtDNA) abundance was quantified as previously described [30] (link). In short, total DNA was extracted from mouse heart tissue lysates, using the QIAamp DSP DNA Mini Kit (Qiagen). Quantitative PCR was performed with mitochondrial DNA- and nuclear DNA-specific primers for COX2 and UCP2 respectively (Table S3), on a Roche Lightcycler 480 using Roche SYBR-green mastermix.
Citrate synthase activity was measured in mouse heart tissue lysates as previously described [31] (link).

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Wijnen W.J., van der Made I., van den Oever S., Hiller M., de Boer B.A., Picavet D.I., Chatzispyrou I.A., Houtkooper R.H., Tijsen A.J., Hagoort J., van Veen H., Everts V., Ruijter J.M., Pinto Y.M, & Creemers E.E. (2014). Cardiomyocyte-Specific miRNA-30c Over-Expression Causes Dilated Cardiomyopathy. PLoS ONE, 9(5), e96290.

Publication 2014

QIAamp DSP DNA Mini Kit Lightcycler 480

Citrate synthase Cox2 Dna primers Heart Mitochondrial dna Mouse Sybr green Tissue

Corresponding Organization : Academic Medical Center

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Mitochondrial DNA (mtDNA) abundance
Citrate synthase activity

control variables

None explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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