After mosquito dissection, protein extraction was carried out separately from the head, thorax with wings, and all available legs according to an earlier protocol (31 (link)). Subsequently, the protein extracts were applied onto a steel plate and coated with an alpha cyano-4-hydroxycinnamic acid matrix. To ensure accuracy and reliability, four technical replicates of each anatomical part were performed for each mosquito specimen.
Protocol detail
Mosquito Protein Extraction Protocol
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Corresponding Organization : Epigénétique et Destin Cellulaire
Other organizations : Cheikh Anta Diop University
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Variable analysis
independent variables
- Anatomical part of mosquito (head, thorax with wings, all available legs)
- Protein expression in different anatomical parts of mosquito
- Extraction protocol
- Protein application and matrix coating on steel plate
- Number of technical replicates (four) for each anatomical part and mosquito specimen
- No positive or negative controls were explicitly mentioned in the input protocol.
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