AMPK Signaling Investigation via Immunoblots

Immunoblots and immunoprecipitation were performed as previously described 16 (link), 19 (link). Rabbit antibodies p-AMPKα (Thr172), AMPKα, Tubulin, acetyl-lysine, LKB1 and horseradish peroxidase-linked secondary antibodies were obtained from Cell Signaling (Danvers, MA, USA). ALDH2 antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The Zeba™ Micro Spin Desalting Columns 7K MWCO (Thermo Fisher, #89877) and Pierce Co-Immunoprecipitation (Co-IP) Kit (Thermo Fisher, #26149) were used for Co-IP experiments.

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Li C., Sun W., Gu C., Yang Z., Quan N., Yang J., Shi Z., Yu L, & Ma H. (2018). Targeting ALDH2 for Therapeutic Interventions in Chronic Pain-Related Myocardial Ischemic Susceptibility. Theranostics, 8(4), 1027-1041.

Publication 2018

Tubulin acetyl-lysine horseradish peroxidase-linked secondary antibodies ALDH2 antibody Zeba™ Micro Spin Desalting Columns 7K MWCO Pierce Co-Immunoprecipitation (Co-IP) Kit

Antibodies Antibody Co immunoprecipitation Horseradish peroxidase Immunoblots Immunoprecipitation Lkb1 Lysine Rabbit Tubulin

Corresponding Organization :

Other organizations : Air Force Medical University, Fu Wai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Xijing Hospital, First Hospital of Jilin University, Jilin University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Phosphorylation of AMPKα (Thr172)
Levels of AMPKα
Levels of Tubulin
Levels of acetyl-lysine
Levels of LKB1
Levels of ALDH2

control variables

None explicitly mentioned

controls

Positive controls: None mentioned
Negative controls: None mentioned

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