Trichome release and enrichment were carried out according to the STIRRER method introduced previously (Huebbers et al. 2022 (link)). Briefly, freshly harvested A. thaliana seedlings were transferred to a 500-mL beaker containing 250 mL of phosphate-buffered saline solution (PBS; 10-mM disodium hydrogen phosphate, 2.7-mM potassium chloride, 2.0-mM potassium dihydrogen phosphate, 0.5-mM magnesium chloride, 137-mM sodium chloride, pH 7.5) supplemented with 50-mM ethylenediaminetetraacetic acid (EDTA). The trichome buffer suspension was stirred at 300 rpm for 30 min. Subsequently, 4 layers of screen door mesh (nominal pore size of 1.2 × 1.4 mm) were used to separate the processed seedlings from the buffer solution containing the released trichomes. The trichomes were captured in a 300-mL beaker and poured through a cell strainer (VWR, Radnor, Pennsylvania, USA) with a nominal pore size of 100 µm. These strained trichomes were collected by a small spatula and transferred to 2-mL reaction tubes. After the first round of agitation and filtration, the process was repeated 2 times for 15 min each. Enriched trichomes were either stored at 4 °C in 1-mL phosphate-buffered saline buffer or frozen in liquid nitrogen and lyophilized for monosaccharide and cellulose quantification or FTIR spectroscopy.