Wound healing assays were performed to detect the migration ability of HNSCC cells. Transwell assays including uncoated inserts and Matrigel‐coated inserts were used separately to determine migration and invasion abilities. The proliferation ability was determined using Cell Counting Kit 8 (CCK8) assays (Dojindo, Japan). Finally, colony‐forming abilities were compared by the number of clusters formed by 1000 cells incubated for 10–14 days. These experiments were performed as described in our previous study and were all repeated three times (Xu et al., 2020 (link)).
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Migration and Invasion Assays for HNSCC
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Other organizations : Beijing Obstetrics and Gynecology Hospital, Capital Medical University
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Variable analysis
independent variables
- Presence of Matrigel coating on transwell inserts
- Migration ability of HNSCC cells
- Invasion ability of HNSCC cells
- Proliferation ability of HNSCC cells
- Colony-forming ability of HNSCC cells
- Cell type (HNSCC cells)
- Number of cells (1000 cells)
- Incubation time (10-14 days)
- Uncoated transwell inserts as a control for migration assay
- Matrigel-coated transwell inserts as a control for invasion assay
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