Adult wild-type and TH-HI mice were randomly assigned to treatment groups that would receive 4 injections of 0.9% saline, 5.0 mg/kg or 10 mg/kg of (+)-Methamphetamine HCl (Sigma; dissolved in 0.9% saline). Treatments were administered through a subcutaneous injection, as previously described (Lohr et al. 2015 (link)). The first dose was administered 60 minutes after baseline core body temperature was measured using a rectal thermometer. Three additional equivalent doses followed, administered at 2-hour intervals (i.e. 180, 300, and 420 minutes following the initial injection). Core body temperature was measured at the time of each injection. Mice were sacrificed by rapid decapitation 48 hours following the first injection, and the striatum was dissected and flash-frozen in liquid nitrogen for protein analyses by Western blot, using standard methods described above. The experimenter was blinded until the point of statistical analyses.