Fundus Fluorescein Angiography in Mice

Fundus fluorescein angiography (FFA) in mice was performed according to a previous study (24 (link)). Mice were anesthetized by ketamine/xylazine intraperitoneal injection and pupils were dilated with topical 1% tropicamide (Santen, Osaka, Japan). Before the procedure, 100 μL of 10% fluorescein sodium (Alcon, US) was injected intraperitoneally. We then placed the mice at a 170° angle with the head closer to the operator. Fluorescent fundus images were acquired within 5 minutes after fluorescein sodium injection. The vascular leakage area of the CNV lesions was determined by measuring the hyperfluorescent area with ImageJ Software.