Antifungal Efficacy Evaluation Protocol

The samples (40 µg/µL) were dissolved in a mixture of dimethylsulfoxide (DMSO) (Sigma-Aldrich, St. Louis, MO, USA) with 0.5% Tween 20. RPMI (Roswell Park Memorial Institute 1640) liquid medium (90 µL) was transferred to each microwell; the ethanol extract (10 µL) and 100 µL of the conidial suspension were added to reach a final ethanolic extract concentration of 2000 µg/mL (Merck Millipore Darmstadt, DE). The negative controls were RPMI (Merck Millipore Darmstadt, DE) (100 µL) and water (100 µL), and a mixture of solvents (0.5% Tween-20 DMSO: RPMI 1:9, v/v) were used. Prochloraz (5 µL) was the positive control, which is described above [29 (link)]. Each sample was performed in triplicate, and all the plates were incubated and assessed as described above.