Two weeks following the last immunization, mice were anesthetized with an intraperitoneal injection of tribromoethanol and then challenged intranasally with either a sublethal dose of 2 × 109 or an abdominally lethal dose of 2 × 108 colony-forming unit (CFU) of A. baumannii WHG40137. The challenge doses were confirmed by CFU counts of serial tenfold dilutions on LB agar. The survival rates of the mice were monitored daily for 7 days. Additionally, bacterial burdens in the lungs and blood of mice with bacterial pneumonia were determined 24 h post the sublethal challenge.
To test the therapeutic effects of the immunized sera, BALB/c mice were anesthetized with tribromoethanol and intranasally challenged with a sublethal dose of 2 × 109 CFU of A. baumannii WHG40137. After 24 h of the infection, 100 μL of either the immunized serum or the control serum was injected into each mouse via tail vein. The bacteria burden in the lungs of the mice was measured by plating serial dilutions on agar plates.
To test the therapeutic effects of the immunized sera, BALB/c mice were anesthetized with tribromoethanol and intranasally challenged with a sublethal dose of 2 × 109 CFU of A. baumannii WHG40137. After 24 h of the infection, 100 μL of either the immunized serum or the control serum was injected into each mouse via tail vein. The bacteria burden in the lungs of the mice was measured by plating serial dilutions on agar plates.
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