Recombinant Neuronal Protein Expression

Ng was cloned by PCR from a commercial rat brain cDNA (Clontech, Mountain View, CA, USA). GFP-Ng was made with pEGFP plasmid and re-cloned into pSinRep5 (Invitrogen, Grand Island, NY, USA) for virus preparation as described (Zhong et al., 2009 (link)). After 5–7 days in culture, GFP-Ng was delivered into the slices using the Sindbis virus expression system, which is a replication-deficient, low-toxicity and neuron-specific system (Malinow et al., 1999 ).

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Zhong L, & Gerges N.Z. (2020). Neurogranin Regulates Metaplasticity. Frontiers in Molecular Neuroscience, 12, 322.

Publication 2019

pSinRep5

Brain Cdna Neuron Plasmid Replication Sindbis virus Virus

Corresponding Organization :

Other organizations : Medical College of Wisconsin

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Variable analysis

independent variables

Delivery of GFP-Ng into the slices using the Sindbis virus expression system

dependent variables

Measurement of the outcomes after delivering GFP-Ng into the slices

control variables

Sindbis virus expression system (replication-deficient, low-toxicity and neuron-specific system)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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