Antiphosphorylated and pan Akt antibodies
were purchased from Cell Signalling Technology (Cambridge, MA, USA).
Interleukin-6 (Il-6) was purchased from Miltenyi Biotec (Auburn, CA,
USA).
The authenticated multiple myeloma-derived cell line MM1
was obtained from the European Collection of Cell Cultures and was
cultured in RPMI 1640 medium supplemented with 10% foetal bovine serum,
penicillin, and streptomycin (all obtained from Invitrogen, Paisley,
UK).44 (link)Cell viability was determined
after 24 h using Cell Titre GLO (Promega,
Southampton, UK).31 (link) Data were normalized
to vehicle controls. All data points are represented as the mean with
s.e.
were purchased from Cell Signalling Technology (Cambridge, MA, USA).
Interleukin-6 (Il-6) was purchased from Miltenyi Biotec (Auburn, CA,
USA).
The authenticated multiple myeloma-derived cell line MM1
was obtained from the European Collection of Cell Cultures and was
cultured in RPMI 1640 medium supplemented with 10% foetal bovine serum,
penicillin, and streptomycin (all obtained from Invitrogen, Paisley,
UK).44 (link)Cell viability was determined
after 24 h using Cell Titre GLO (Promega,
Southampton, UK).31 (link) Data were normalized
to vehicle controls. All data points are represented as the mean with
s.e.
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