As previously described 7 (link),8 (link),33 (link), mice were immunized with an intraperitoneal injection of 10 mg ovalbumin (OVA) (Sigma-Aldrich, St. Louis, MO, USA) in 300 ng pertussis toxin (Sigma-Aldrich) and 1 mg aluminum hydroxide (Thermo Fisher Scientific, Waltham, MA, USA) in PBS. After a 14 day rest period, mice were given topical application of 250 mg OVA solution (i.e. OVA challenged) once/day to each eye for at least 7 days. Naïve mice did not receive the OVA challenge.
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Ovalbumin-Induced Allergic Conjunctivitis
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Corresponding Organization : Massachusetts Eye and Ear Infirmary
Other organizations : Duke University, University of Oslo, Oslo University Hospital, Brigham and Women's Hospital
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Variable analysis
independent variables
- Intraperitoneal injection of 10 mg ovalbumin (OVA), 300 ng pertussis toxin, and 1 mg aluminum hydroxide in PBS
- Topical application of 250 mg OVA solution to each eye for at least 7 days
- Outcome measures not explicitly mentioned
- Naïve mice that did not receive the OVA challenge
- Positive control: Mice that received the intraperitoneal injection and OVA challenge
- Negative control: Naïve mice that did not receive the OVA challenge
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