MBC was determined by plating a 10 μL aliquot on solid culture Mueller–Hinton medium (Oxoid Ltd., Basingstoke, Hampshire, UK) from the last 4 wells that showed inhibition of bacterial growth in the MIC testing. The plates were incubated for 24 h at 37 °C. The lowest concentration that prevented the growth of bacteria (no colonies on the plate) was considered the MBC. Three different biological replicates were performed for each plate [38 (link)].
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