Conditional Deletion of Wnt Signaling Regulator

The following transgenic mice used in this study were generated previously: EIIa-cre (Lakso et al., 1996 (link)), Wnt1-cre (Danielian et al., 1998 (link)), TOPGAL (DasGupta and Fuchs, 1999 (link)), and Pdx1-cre (Wells et al., 2007 (link)). The Wls floxed mice were crossed to the EIIa-cre, Wnt1-cre, and Pdx1-cre mice to generate conditional mice where Wls would be deleted in the germline, neural crest cells, or pancreatic progenitor cells, respectively. All mouse lines used in this study were genotyped by PCR using primers and protocols described previously. Yolk sacs from staged embryos or tail tips were digested overnight at 55°C in lysis buffer and genomic DNA extracted using a Kingfisher 96 Magnetic Particle Processor.

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Carpenter A.C., Rao S., Wells J.M., Campbell K, & Lang R.A. (2010). Generation of Mice with a Conditional Null Allele for Wntless. Genesis (New York, N.Y. : 2000), 48(9), 554-558.

Publication 2010

Buffer Embryos yolk sacs Genomic Germline Mouse Neural crest cells Pancreatic Pdx1 Primers Progenitor cells Tail Transgenic mice Wnt1

Corresponding Organization : University of Cincinnati

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Variable analysis

independent variables

Crossing of Wls floxed mice to EIIa-cre, Wnt1-cre, and Pdx1-cre mice to generate conditional mice where Wls would be deleted in the germline, neural crest cells, or pancreatic progenitor cells, respectively

dependent variables

Not explicitly mentioned

control variables

All mouse lines used in this study were genotyped by PCR using primers and protocols described previously
Yolk sacs from staged embryos or tail tips were digested overnight at 55°C in lysis buffer and genomic DNA extracted using a Kingfisher 96 Magnetic Particle Processor

controls

No positive or negative controls were explicitly mentioned

Annotations

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