Cells were routinely grown in LB medium containing 1% Bacto Tryptone (Difco), 0.5% yeast extract (Difco), and 0.5% NaCl with or without antibiotics at 50 μg/ml for ampicillin (Wako, Osaka, Japan) and 30 μg/ml for kanamycin (Wako, Osaka, Japan). Glucose, L -arabinose, and other chemicals were from Wako (Osaka, Japan). DpnI was from New England Biolabs (MA, USA); Taq polymerase, TaKaRa Ex Taq, and agarose, SeaKem GTG Agarose from Takara Shuzo Inc. E-Gel 96 systems were from Invitrogen. MOPS medium was prepared as described elsewhere (Wanner, 1994 ).
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Bacterial Cell Culture and Genetic Manipulation
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Corresponding Organization :
Other organizations : Keio University, Nara Institute of Science and Technology, Japan Science and Technology Agency, Purdue University West Lafayette
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Variable analysis
independent variables
- Presence or absence of antibiotics (ampicillin at 50 μg/ml or kanamycin at 30 μg/ml)
- Not explicitly mentioned
- Cell growth in LB medium containing 1% Bacto Tryptone, 0.5% yeast extract, and 0.5% NaCl
- Positive control: Cells grown with antibiotics
- Negative control: Cells grown without antibiotics
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